				Packard 
			A BioScience Company
			Certification of Quality
		  	OptiQuant for NT Master CD
		        PPN 7800396/F
			Version 4.00 
		---------------------------------
              
                        
Packard Instrument Company guarantees that this software product is 
designed, tested and manufactured in accordance with our procedures 
which are certified under ISO 9001.  Documentation of the procedures 
used, and software reliability reports are available for this and any 
other software products upon request. 
Requests for these documents should be made in writing to:  

		Packard Instrument Company
		Attn: Quality Assurance Dept.
		2200 Warrenville Road
		Downers Grove, IL 60515

or by FAX to:       (630) 969-6511

Please include the serial number of the unit for which the request is 
made, along with the Packard Part Number (PPN) and version number of 
the Software Master Disks installed in the unit. 
 

OptiQuant/Cyclone Software Installation
[NOTE: SCSI Board and drivers must be installed before this installation.]

1. Insert " OptiQuant CD" in CD drawer.
2. After "OptiQuant  Setup" screen comes up Select " Next ".
	a. Select "Next"
	b. Select "Next" for Destination Folder to install on drive C:
	c. Select "Next" to install to " OptiQuant" folder.
	d. Select " Yes" if you want to install the "Cyclone" software.
3. Select " Next " in the "Cyclone Setup" screen.
	a. Select " Next " 
	b. Select " OK " if information message comes up to update SCSI Drivers "
		[ NOTE This screen will only come up if an update is necessary.]
		1. Select "Yes" to continue ASPI upgrade.
		2. Select "Upgrade"
		3. Select "Reboot"
4. Leave CD in CD Drawer.
5.After computer reboots, Cyclone Setup will continue
	a. Select "Next"
	b. Select "Next" to continue.
	c. Select "Finish" to complete setup.



             

        
	

   

Version 4.00 Enhancements
	1. Add support for Windows NT 4.00  
--------------------------------------------------------------

Version 3.10 Enhancements
	1. Corrected Lane Baseline background subtract computations
	 for rotated lanes.

Version 3.00 Enhancements
  1)  Added Line, Freehand and Text Annotations
  2)  Added Eraser Tool for Annotations
  3)  Installation modified to allow uninstall from Windows/95
  4)  Right Mouse Button context menus added for Templates, Regions, 
        Annotations and Image.
  5)  Copy/Paste items added to all toolbars and context menus
  6)  Printing enhancements:
	a. Allow printing on multiple pages.
	b. Speed up printing of images .
	c. Heading in reports in BOLD type
	d. Added Underline below heading and  page number in reports .
	e. Allow Print Preview with magnification .
	f. Allow Print Preview of selected region.

 7)  Image Stitching - Allow stitched image to be moved above or to
        the left of stationary image.
 8)  Image Arithmetic - New dialog with Pixel Offset button.
	a. Changed final result to only add  the overlapping areas.
 9)  Image Overlay	- New dialog with pixel offset button
10)  Image Overlay - Images positioned based on order in dialog box.
11)  Image Information 
	a. Added Image Alteration field
	b. Orientation more descriptive
	c. Photometric Interpretation more descriptive.

12)  Lane Profiles
	a. Added Image display in profile view.
	b. Added Peak Tags (Type, orientation and Font).
	c. Added more menu items in the Right Mouse Context Menu.
	d. Split/Merge tool grayed out if template selected.
	e. Overlaid plots implemented.
13)  Preferences
	a. Added ability to specify colors for overlaid plots.
14)  Miscellaneous
	a. Add print preview button to file toolbar.
	b. Changed bitmap for Packard logo.
	c. Fix floating toolbar problem.
	d. Updated registry to include report items not saved.
	e. Added image offset to registry.
	f. Added profile plot colors to registry.
15)  When creating a rectangular or elliptical region, holding down
        the SHIFT key will maintain a 1:1 aspect ratio.
16)  Added magnify tool to profile view
17)  Added Hand tool to profile view
18)  Modified target directory to use user's directory for Export,
       Flip,Rotate etc.
19)  Numbered Untitled files 
20)  Added the Delete button to Image View and Profile view.
21)  Changed Install to include DCOM95 .


Version 3.00 Modifications
---------------------------------
 1)  Changed resizing Ques to  red.
 2)  Changed resizing que for individual lane width to <-> .
 3)  Changed Variable print factors to default to whatever settings
      THAT USER used the last time printed.
 4)  Save specified values for DLU Units and DLU /mm2 Units  with template .
 5)  Deleting User  deletes User Icon without the need of rebooting the computer .
 6)  Help added for "Clipboard and Close" in Reports .
 7)  Help added in " Print " dialog box .
 8)  Allow uninstall Cyclone 3.0 and OptiQuant 3.0 using Uninstall 
      from "Add/ Remove Programs" in " Control Panel". 
 9)  Improve Image Printout in Landscape mode .
10) Do not close images when using Image Arithmetic using "Cancel"
       if the chosen images have templates and reports on them .
11) Changes made in Image Arithmetic after preview registration do not
       require new preview registrations after a color is changed on the images.
12) Do not allow entry of random values for Quota in User Properties.
13) Allow the creation of a 1 pixel wide region using mouse click in lane profile view.
14) Save Stitched images to users directory.
15) Sync scrolling hand motion with all the images in view when
       using Image Arithmetic and Image Overlay .

		  
Version 2.50 Enhancements
-------------------------
	
1)  Added Image Overlay feature.
   
2)  Added Image Arithmetic feature.

3)  Added Copy/Paste of templates and regions.

4)  Added Image Information Dialog box with print option.

5)  Background Color configurable via Dialog box in Preferences
     menu selection.

6)  Added Optical Density Dialog box in Transform menu which
     will allow you to specify optical density transformation 
     parameters of OptiQuant.( Not used for Cyclone.) 

7)  Added the template number (i.e. T1,T2,etc.)to heading in 
     Grid ,Lane Region and Group of Regions, Edit dialog boxes.
	
8)  Reports display headers on every page in Print and Print 
     Preview.

9)  Added Image Offset feature.

10) Installation for OptiQuant and Cyclone separated.

11) Improved printout of images in Reports .
      a)  Improved gray scale printout.
      b)  Template tags and artifacts printout improved .


Version 2.50 Modifications
----------------------------------

1)  Automatically close irregular regions.
	
2)  Report with one template now skips template selection dialog. 

3)  Font selection available in report window.

4)  Lane Toolbox tools now available when Lane template is 
      selected.

5)  Color of tool changes for multiple selection.

6)  Distance to origin is displayed when a Lane template is
     selected.

7)  Regions now are assigned to the proper lane.

8)  Added graphical border around image.

9)  Blanked out Width & Lane Spacing in Lane Template edit 
     dialog, when template is edited graphically resulting in
     different values for Lane Width or Lane Spacing ..

10) Show decimals in Display palette and Manual Rescale 
      Dialog Box.

11) Confirm deletion of User accounts.

12) Disable Start scan of Cyclone, when no scan area is defined.

13) Added Cursor for profile drawing tools.

14) Save Max. scan area for all carousels.

15) Added Cancel button for color dialog box.

16) Displayed maximum value now the same as defined value.

17) When a region is deleted from a template ,focus will shift 
      to next region within template. 

18) Images will retain same magnification of closed image 
      when reopened .

19) Images with templates will have focus on T1 when opened.

20) Template, lane, region and label boxes in template bar 
      made inactive, when no template present on image.

21) In Reports "Fonts Style" - italic, bold, etc. now operational.

22) Correct file structure when saving reports as WK1 files.


    Special Notes:
    --------------

I) Acquiring Images:  
	A) The Cyclone requires a large number of resources from the
	      System to successfully acquire the image.
	      Please refrain from performing intensive tasks during 
	      the acquisition, as the SCAN MIGHT BE ABORTED 
	      if resources are not available.

	   1) Do Not Print while acquiring.

	   2) Must have 80 Meg of Drive Space Free  to let an 
	       Acquisition begin.
		a) Note: This includes the space required by Windows 95
		 swap file ( WIN386.SWP )which increases in size as 
                                applications are opened.
		
		b) Note: A 600 DPI Long Carousel Image requires almost
		    60 Meg of drive space.		

	   3) Only one User can acquire an image at a time.

	   4) Rotating and Flipping Images:
	       An error may occur when rotating and flipping large
		 images when system drive space becomes too small.
		 Check drive space before starting .

	   5) Generating Template Reports:
	        If grid template reports get too large, the Swap file
		 will get excessively large and may stop acquisition .

	   6) Do not open any memory intensive applications while
              acquiring.

	   7) Do not use the CD ROM while acquiring .

	   8) Do not install other software during acquisition .
	   

	   9) Networks: 
	       Do not acquire Cyclone images directly to a network 
	       drive.
	      
	   10) Jaz and Zip Drives: 
	         Do not acquire Cyclone images directly to a Jaz or
		 Zip Drive, time-out errors will result, causing
		 scans to abort.

 II) Reducing Communication Errors:


	A) Power Managers:
	     Disable Windows 95 Power Managers to prevent Cyclone 
	     Communication Errors when System is inactive.

	   1) Windows 95 4.00.950 and Windows 95 4.00.950 A
		 a) From Control Panel select " Power " icon.
		 b) Select "OFF " from " Power Management ".
		 c) Select " Apply ".
		 d) Select " OK ".

	   2) Windows 95 4.00.950 B
		 a) From Control Panel select  " Power " Icon.
		 b) Select " Disk Drives ".
		 c) Uncheck " When Powered by AC Power ".
		 d) Select " Power ".
		 e) Uncheck " Allow Windows to Manage Power Use on
		    this Computer ".
		 f) Select " Apply ".
		 g) Select  " Yes " To stop Power Management.
		 h) Select  " Yes " To Restart Computer. 

            * If no " Power " Icon exists, the Power management is
              disabled in the system BIOS -- no action required. 
 
	B) Optimizing Drive Space:
	   1) Archive and delete old files and images .
	   2) Delete *.Tmp files from C:\Windows\Temp directory.
	   3) Empty " Recycle Bin " 
		   a) Change Properties of " Recycle Bin ".
			1. Right Click " Recycle Bin " Icon
			2. Select Global.
			3. Move Slider to the left ( 1% ) . 
	   4) Scan and Defragment hard drive to regain lost space
		   and make hard drive access faster .

	C) Disable Screen Savers.

	D) Follow proper power up sequence :
	   1) Turn on Cyclone.
	   2) Turn on Computer.
	
	E) Note: If power is lost on the Cyclone even when an acquire
	     is not taking place a Communication error will result.
		a) Will have to shut down and follow the power up
		 sequence.

	F) Close down as many applications as possible before starting
	     an Acquisition . ( Be sure to wait until Swap file is
	     resized about 10 to 30 seconds ).
	
	G) System Memory - 32 Meg minimum recommended for good 
	     results. Note: When system memory is used up by 
	     applications Windows 95 will use Drive C: Swap
	    file as virtual memory.
	   	a. If your applications are large add more memory .
		b. Free up Drive Space for Swap file .

	H) Restart the computer before installing or removing any 
	   software applications . This will keep any active
 	   applications from interfering with your operation .
		a. Note : This will require following the power up
 		  sequence for the Cyclone and computer.	           


III) Video:
	For optimal results use these display modes: 
	800 X 600 or 1024 X 768 
	256 color with Small Fonts.

 	Large Fonts may produce some cropping at the bottom of
	the display.
	640 X 480 may produce some cropping of display.
	When using 24 bit or 32 bit color , Image magnification
 	may get distorted when 6400 % magnification is reached .
	To correct this problem demagnify image back to 3200 % .

IV)  Iomega Zip Drive: 
	To prevent possible lock ups while printing an image 
	from a Zip Drive. Set the " Spool Settings " on HP 
	Printer Properties to " Start Printing After Last
	Page is Spooled " . 
  
V)   Stitching Images:                                         
	When stitching creates a resulting image greater than 1 
	meter in length or width, only the top left 1 meter 
	square will be accessible.
    
VI)  Rotating Images:
	An error may occur when rotating large images when system 
	drive space becomes too small. Check drive space before 
	rotation of large images.
    
VII) Rev 1.00 and 2.00 File Compatibility:
	A)Image templates and image files which have been saved 
	    in Version 3.00 can not be used in Version 1.00 ,2.00 
	    or 2.50 of OptiQuant. All standalone versions of
	    OptiQuant should be upgraded to version 3.00. 

	B)Infrequent problems when opening or saving certain .ANA 
	    files which were created using OptiQuant 1.00 on a 
	    Cyclone.  (.ANA files are analysis files associated with 
	    image files with the same filename).
	    - Only files which have no templates defined will show this 
	      problem.
	    - The problem can occur in two places:
	    - When opening an image with the corresponding .ANA file.
	    - When performing a Save Template operation on a 
	       successfully opened file.
	    - Windows 95 must be restarted when this problem occurs.
	    - If the .ANA file is deleted, the image will always open 
	      successfully.
	    - Once the .ANA file has been saved with OQ 3.00, it will 
	      always open successfully.

VIII) Image printouts:
	A) HP LaserJet 4000 PCL 6 - Drivers may cause a software lockup.
	     Use HP LaserJet PCL 5e Drivers only.
	     Use Control Panel to set HP LaserJet PCL 5e as Default Printer.
	   
	B) Enhanced Printer Drivers:
	     Printing images in "Landscape" using HP LaserJet 5 Enhanced
	     Printer Drivers may result in improper print out.
	     Use HP LaserJet Standard Drivers or print in "Portrait" mode .
	
IX)   Importing certain 8-bit TIFFs:
	OptiQuant will always open TIFF files expecting them to 
	contain quantifiable data.  Some 8-bit TIFFs (including 
	OptiQuant's own exported images) are intended to be used 
	as visual representations only.  These imported files may 
	not be displayed as expected, especially at the high and 
	low intensity values.

X)    Lane background subtract:
	Computations for background subtraction are slightly 
	different when background is defined with regions as 
	compared to backgrounds defined with a baseline. The
	baseline method varies as a result of fractions of 
	pixels at the end of a lane.

XI)   Zoom is limited by image size.  Crop to create a smaller 
	image to zoom in closer.

XII)  Cancel button in "instrument detection" dialog box does not 
	stop detection.  It only allows users to perform other tasks 
	while diagnostics are in progress.

XIII) Image Overlay contrast may be low when using images with a 
	low palette intensity range.

XIV) Infrequent problem may occur when acquisition is finished .
	The acquire dialog remains open and appears to be busy.
	Use the "X" button on the top right of "Cyclone Control Room"
	task bar to close down dialog box. 
	To start a new acquisition select " Instrument "

XV)  Errors  with Open Files when removable media is removed:
	Be sure to close any files which were saved to or opened from
	removable media ( Floppy, Zip Drive or Jaz Drive etc.) before
	removing the media . 
 
XVI) Infrequent problem may occur when an acquisition is taking
	place, and a second user tries to access acquisition. When
	second user closes the acquisition dialog  an error may occur 
	which closes the second user's session  of  OptiQuant .
	    

          

	    






